mhc type iix muscle fibers Search Results


mhc iix  (Developmental Studies Hybridoma Bank)
99
Developmental Studies Hybridoma Bank mhc iix
Surviving Spin1 M5 mice exhibit major defects in soleus, tibialis anterior, and diaphragm. ( a,b ) Appearance ( a ) and average body weight ( b ) of Spin1 M5 and control mice ( n =5 females in each category) at 30 weeks of age. Error bars represent +S.D., ** P <0.01. ( c ) Degeneration of the soleus (SOL) muscle in adult Spin1 M5 compared with control mice exemplified at 16 weeks of age. ( d ) Hind limb muscles (gastrocnemius (GC), plantaris (PL), soleus, tibialis anterior (TA), extensor digitorum longus (EDL), and quadriceps (QC)) of Spin1 M5 and control mice at 30 weeks of age. Arrows point at the soleus embedded in gastrocnemius and plantaris, which is visible in control but degenerated in Spin1 M5 mice. ( e ) Hematoxylin & eosin (H&E) staining of gastrocnemius, soleus, TA, and EDL muscle of Spin1 M5 and control mice at 30 weeks of age. ( f ) Fiber types in glycolytic (white) or oxidative (red) parts of the TA of Spin1 M5 and control mice at 15 weeks of age (top and middle rows) observed by immunofluorescence (IF) staining. Tissue sections were stained with selective antibody directed against <t>MHC-I</t> (purple), MHC-IIb <t>(cyan),</t> <t>MHC-IIa</t> (red), and MHC-IIx (green). For comparison, NADH staining was included (bottom row). Fibers with abnormal NADH staining are marked with arrows. Corresponding fibers in each column of images are squared
Mhc Iix, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti myosin heavy chain  (Miltenyi Biotec)
91
Miltenyi Biotec anti myosin heavy chain
Surviving Spin1 M5 mice exhibit major defects in soleus, tibialis anterior, and diaphragm. ( a,b ) Appearance ( a ) and average body weight ( b ) of Spin1 M5 and control mice ( n =5 females in each category) at 30 weeks of age. Error bars represent +S.D., ** P <0.01. ( c ) Degeneration of the soleus (SOL) muscle in adult Spin1 M5 compared with control mice exemplified at 16 weeks of age. ( d ) Hind limb muscles (gastrocnemius (GC), plantaris (PL), soleus, tibialis anterior (TA), extensor digitorum longus (EDL), and quadriceps (QC)) of Spin1 M5 and control mice at 30 weeks of age. Arrows point at the soleus embedded in gastrocnemius and plantaris, which is visible in control but degenerated in Spin1 M5 mice. ( e ) Hematoxylin & eosin (H&E) staining of gastrocnemius, soleus, TA, and EDL muscle of Spin1 M5 and control mice at 30 weeks of age. ( f ) Fiber types in glycolytic (white) or oxidative (red) parts of the TA of Spin1 M5 and control mice at 15 weeks of age (top and middle rows) observed by immunofluorescence (IF) staining. Tissue sections were stained with selective antibody directed against <t>MHC-I</t> (purple), MHC-IIb <t>(cyan),</t> <t>MHC-IIa</t> (red), and MHC-IIx (green). For comparison, NADH staining was included (bottom row). Fibers with abnormal NADH staining are marked with arrows. Corresponding fibers in each column of images are squared
Anti Myosin Heavy Chain, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mhc type iix muscle fibers  (Developmental Studies Hybridoma Bank)
99
Developmental Studies Hybridoma Bank mhc type iix muscle fibers
Surviving Spin1 M5 mice exhibit major defects in soleus, tibialis anterior, and diaphragm. ( a,b ) Appearance ( a ) and average body weight ( b ) of Spin1 M5 and control mice ( n =5 females in each category) at 30 weeks of age. Error bars represent +S.D., ** P <0.01. ( c ) Degeneration of the soleus (SOL) muscle in adult Spin1 M5 compared with control mice exemplified at 16 weeks of age. ( d ) Hind limb muscles (gastrocnemius (GC), plantaris (PL), soleus, tibialis anterior (TA), extensor digitorum longus (EDL), and quadriceps (QC)) of Spin1 M5 and control mice at 30 weeks of age. Arrows point at the soleus embedded in gastrocnemius and plantaris, which is visible in control but degenerated in Spin1 M5 mice. ( e ) Hematoxylin & eosin (H&E) staining of gastrocnemius, soleus, TA, and EDL muscle of Spin1 M5 and control mice at 30 weeks of age. ( f ) Fiber types in glycolytic (white) or oxidative (red) parts of the TA of Spin1 M5 and control mice at 15 weeks of age (top and middle rows) observed by immunofluorescence (IF) staining. Tissue sections were stained with selective antibody directed against <t>MHC-I</t> (purple), MHC-IIb <t>(cyan),</t> <t>MHC-IIa</t> (red), and MHC-IIx (green). For comparison, NADH staining was included (bottom row). Fibers with abnormal NADH staining are marked with arrows. Corresponding fibers in each column of images are squared
Mhc Type Iix Muscle Fibers, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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genome analyzer iix  (Illumina Inc)
90
Illumina Inc genome analyzer iix
Surviving Spin1 M5 mice exhibit major defects in soleus, tibialis anterior, and diaphragm. ( a,b ) Appearance ( a ) and average body weight ( b ) of Spin1 M5 and control mice ( n =5 females in each category) at 30 weeks of age. Error bars represent +S.D., ** P <0.01. ( c ) Degeneration of the soleus (SOL) muscle in adult Spin1 M5 compared with control mice exemplified at 16 weeks of age. ( d ) Hind limb muscles (gastrocnemius (GC), plantaris (PL), soleus, tibialis anterior (TA), extensor digitorum longus (EDL), and quadriceps (QC)) of Spin1 M5 and control mice at 30 weeks of age. Arrows point at the soleus embedded in gastrocnemius and plantaris, which is visible in control but degenerated in Spin1 M5 mice. ( e ) Hematoxylin & eosin (H&E) staining of gastrocnemius, soleus, TA, and EDL muscle of Spin1 M5 and control mice at 30 weeks of age. ( f ) Fiber types in glycolytic (white) or oxidative (red) parts of the TA of Spin1 M5 and control mice at 15 weeks of age (top and middle rows) observed by immunofluorescence (IF) staining. Tissue sections were stained with selective antibody directed against <t>MHC-I</t> (purple), MHC-IIb <t>(cyan),</t> <t>MHC-IIa</t> (red), and MHC-IIx (green). For comparison, NADH staining was included (bottom row). Fibers with abnormal NADH staining are marked with arrows. Corresponding fibers in each column of images are squared
Genome Analyzer Iix, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti pan myhc except iix  (Developmental Studies Hybridoma Bank)
96
Developmental Studies Hybridoma Bank anti pan myhc except iix
Surviving Spin1 M5 mice exhibit major defects in soleus, tibialis anterior, and diaphragm. ( a,b ) Appearance ( a ) and average body weight ( b ) of Spin1 M5 and control mice ( n =5 females in each category) at 30 weeks of age. Error bars represent +S.D., ** P <0.01. ( c ) Degeneration of the soleus (SOL) muscle in adult Spin1 M5 compared with control mice exemplified at 16 weeks of age. ( d ) Hind limb muscles (gastrocnemius (GC), plantaris (PL), soleus, tibialis anterior (TA), extensor digitorum longus (EDL), and quadriceps (QC)) of Spin1 M5 and control mice at 30 weeks of age. Arrows point at the soleus embedded in gastrocnemius and plantaris, which is visible in control but degenerated in Spin1 M5 mice. ( e ) Hematoxylin & eosin (H&E) staining of gastrocnemius, soleus, TA, and EDL muscle of Spin1 M5 and control mice at 30 weeks of age. ( f ) Fiber types in glycolytic (white) or oxidative (red) parts of the TA of Spin1 M5 and control mice at 15 weeks of age (top and middle rows) observed by immunofluorescence (IF) staining. Tissue sections were stained with selective antibody directed against <t>MHC-I</t> (purple), MHC-IIb <t>(cyan),</t> <t>MHC-IIa</t> (red), and MHC-IIx (green). For comparison, NADH staining was included (bottom row). Fibers with abnormal NADH staining are marked with arrows. Corresponding fibers in each column of images are squared
Anti Pan Myhc Except Iix, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sc-71 antibody  (Developmental Studies Hybridoma Bank)
90
Developmental Studies Hybridoma Bank sc-71 antibody
Surviving Spin1 M5 mice exhibit major defects in soleus, tibialis anterior, and diaphragm. ( a,b ) Appearance ( a ) and average body weight ( b ) of Spin1 M5 and control mice ( n =5 females in each category) at 30 weeks of age. Error bars represent +S.D., ** P <0.01. ( c ) Degeneration of the soleus (SOL) muscle in adult Spin1 M5 compared with control mice exemplified at 16 weeks of age. ( d ) Hind limb muscles (gastrocnemius (GC), plantaris (PL), soleus, tibialis anterior (TA), extensor digitorum longus (EDL), and quadriceps (QC)) of Spin1 M5 and control mice at 30 weeks of age. Arrows point at the soleus embedded in gastrocnemius and plantaris, which is visible in control but degenerated in Spin1 M5 mice. ( e ) Hematoxylin & eosin (H&E) staining of gastrocnemius, soleus, TA, and EDL muscle of Spin1 M5 and control mice at 30 weeks of age. ( f ) Fiber types in glycolytic (white) or oxidative (red) parts of the TA of Spin1 M5 and control mice at 15 weeks of age (top and middle rows) observed by immunofluorescence (IF) staining. Tissue sections were stained with selective antibody directed against <t>MHC-I</t> (purple), MHC-IIb <t>(cyan),</t> <t>MHC-IIa</t> (red), and MHC-IIx (green). For comparison, NADH staining was included (bottom row). Fibers with abnormal NADH staining are marked with arrows. Corresponding fibers in each column of images are squared
Sc 71 Antibody, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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myhc iix 6h1  (Developmental Studies Hybridoma Bank)
99
Developmental Studies Hybridoma Bank myhc iix 6h1
(A) Morphology of the gastrocnemius of wild-type (WT) and HSA-NRTN mice. (B) Weight of soleus, extensor digitorum longus (EDL), gastrocnemius, and tibialis anterior (TA) muscles (n = 9-12) normalized by body weight. (C) Histology of WT and HSA-NRTN TA muscles as determined by hematoxylin and eosin staining. (D and E) Fiber cross-sectional area (CSA) distribution (D) and average (E) in TA muscles of WT and HSA-NRTN mice (n = 5-7). (F and G) Immunohistochemical analysis of fiber type composition in TA muscles of WT and HSA-NRTN mice. Cell membranes are visualized using wheat germ agglutinin (WGA, in red). Representative microscopy images (F) and the corresponding quantification (G) are shown. (H) <t>MyHC-IIx</t> and MyHC-IIb protein levels in gastrocnemius muscles of WT and HSA-NRTN mice. (I) Gene expression of calcium handling machinery components. The graph shows the relative expression of genes involved in calcium handling in gastrocnemius muscles of wild-type and HSA-NRTN mice. Each gene is annotated as enriched in fast muscle (EDL) or slow muscle (soleus) based on published gene array data comparing mouse EDL and soleus muscles (n = 6). Bars depict mean values and error bars represent SEM. * p<0.05, ** p<0.01, *** p<0.001. See also Figure S2 .
Myhc Iix 6h1, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp myh1 mm01332489 m1  (Thermo Fisher)
99
Thermo Fisher gene exp myh1 mm01332489 m1
(A) Morphology of the gastrocnemius of wild-type (WT) and HSA-NRTN mice. (B) Weight of soleus, extensor digitorum longus (EDL), gastrocnemius, and tibialis anterior (TA) muscles (n = 9-12) normalized by body weight. (C) Histology of WT and HSA-NRTN TA muscles as determined by hematoxylin and eosin staining. (D and E) Fiber cross-sectional area (CSA) distribution (D) and average (E) in TA muscles of WT and HSA-NRTN mice (n = 5-7). (F and G) Immunohistochemical analysis of fiber type composition in TA muscles of WT and HSA-NRTN mice. Cell membranes are visualized using wheat germ agglutinin (WGA, in red). Representative microscopy images (F) and the corresponding quantification (G) are shown. (H) <t>MyHC-IIx</t> and MyHC-IIb protein levels in gastrocnemius muscles of WT and HSA-NRTN mice. (I) Gene expression of calcium handling machinery components. The graph shows the relative expression of genes involved in calcium handling in gastrocnemius muscles of wild-type and HSA-NRTN mice. Each gene is annotated as enriched in fast muscle (EDL) or slow muscle (soleus) based on published gene array data comparing mouse EDL and soleus muscles (n = 6). Bars depict mean values and error bars represent SEM. * p<0.05, ** p<0.01, *** p<0.001. See also Figure S2 .
Gene Exp Myh1 Mm01332489 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a4 74  (Developmental Studies Hybridoma Bank)
99
Developmental Studies Hybridoma Bank a4 74
(A) Morphology of the gastrocnemius of wild-type (WT) and HSA-NRTN mice. (B) Weight of soleus, extensor digitorum longus (EDL), gastrocnemius, and tibialis anterior (TA) muscles (n = 9-12) normalized by body weight. (C) Histology of WT and HSA-NRTN TA muscles as determined by hematoxylin and eosin staining. (D and E) Fiber cross-sectional area (CSA) distribution (D) and average (E) in TA muscles of WT and HSA-NRTN mice (n = 5-7). (F and G) Immunohistochemical analysis of fiber type composition in TA muscles of WT and HSA-NRTN mice. Cell membranes are visualized using wheat germ agglutinin (WGA, in red). Representative microscopy images (F) and the corresponding quantification (G) are shown. (H) <t>MyHC-IIx</t> and MyHC-IIb protein levels in gastrocnemius muscles of WT and HSA-NRTN mice. (I) Gene expression of calcium handling machinery components. The graph shows the relative expression of genes involved in calcium handling in gastrocnemius muscles of wild-type and HSA-NRTN mice. Each gene is annotated as enriched in fast muscle (EDL) or slow muscle (soleus) based on published gene array data comparing mouse EDL and soleus muscles (n = 6). Bars depict mean values and error bars represent SEM. * p<0.05, ** p<0.01, *** p<0.001. See also Figure S2 .
A4 74, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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main skeletal muscle mhc isoforms  (Developmental Studies Hybridoma Bank)
99
Developmental Studies Hybridoma Bank main skeletal muscle mhc isoforms
(A) Morphology of the gastrocnemius of wild-type (WT) and HSA-NRTN mice. (B) Weight of soleus, extensor digitorum longus (EDL), gastrocnemius, and tibialis anterior (TA) muscles (n = 9-12) normalized by body weight. (C) Histology of WT and HSA-NRTN TA muscles as determined by hematoxylin and eosin staining. (D and E) Fiber cross-sectional area (CSA) distribution (D) and average (E) in TA muscles of WT and HSA-NRTN mice (n = 5-7). (F and G) Immunohistochemical analysis of fiber type composition in TA muscles of WT and HSA-NRTN mice. Cell membranes are visualized using wheat germ agglutinin (WGA, in red). Representative microscopy images (F) and the corresponding quantification (G) are shown. (H) <t>MyHC-IIx</t> and MyHC-IIb protein levels in gastrocnemius muscles of WT and HSA-NRTN mice. (I) Gene expression of calcium handling machinery components. The graph shows the relative expression of genes involved in calcium handling in gastrocnemius muscles of wild-type and HSA-NRTN mice. Each gene is annotated as enriched in fast muscle (EDL) or slow muscle (soleus) based on published gene array data comparing mouse EDL and soleus muscles (n = 6). Bars depict mean values and error bars represent SEM. * p<0.05, ** p<0.01, *** p<0.001. See also Figure S2 .
Main Skeletal Muscle Mhc Isoforms, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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iix muscle fibers  (Developmental Studies Hybridoma Bank)
99
Developmental Studies Hybridoma Bank iix muscle fibers
(A) Morphology of the gastrocnemius of wild-type (WT) and HSA-NRTN mice. (B) Weight of soleus, extensor digitorum longus (EDL), gastrocnemius, and tibialis anterior (TA) muscles (n = 9-12) normalized by body weight. (C) Histology of WT and HSA-NRTN TA muscles as determined by hematoxylin and eosin staining. (D and E) Fiber cross-sectional area (CSA) distribution (D) and average (E) in TA muscles of WT and HSA-NRTN mice (n = 5-7). (F and G) Immunohistochemical analysis of fiber type composition in TA muscles of WT and HSA-NRTN mice. Cell membranes are visualized using wheat germ agglutinin (WGA, in red). Representative microscopy images (F) and the corresponding quantification (G) are shown. (H) <t>MyHC-IIx</t> and MyHC-IIb protein levels in gastrocnemius muscles of WT and HSA-NRTN mice. (I) Gene expression of calcium handling machinery components. The graph shows the relative expression of genes involved in calcium handling in gastrocnemius muscles of wild-type and HSA-NRTN mice. Each gene is annotated as enriched in fast muscle (EDL) or slow muscle (soleus) based on published gene array data comparing mouse EDL and soleus muscles (n = 6). Bars depict mean values and error bars represent SEM. * p<0.05, ** p<0.01, *** p<0.001. See also Figure S2 .
Iix Muscle Fibers, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Surviving Spin1 M5 mice exhibit major defects in soleus, tibialis anterior, and diaphragm. ( a,b ) Appearance ( a ) and average body weight ( b ) of Spin1 M5 and control mice ( n =5 females in each category) at 30 weeks of age. Error bars represent +S.D., ** P <0.01. ( c ) Degeneration of the soleus (SOL) muscle in adult Spin1 M5 compared with control mice exemplified at 16 weeks of age. ( d ) Hind limb muscles (gastrocnemius (GC), plantaris (PL), soleus, tibialis anterior (TA), extensor digitorum longus (EDL), and quadriceps (QC)) of Spin1 M5 and control mice at 30 weeks of age. Arrows point at the soleus embedded in gastrocnemius and plantaris, which is visible in control but degenerated in Spin1 M5 mice. ( e ) Hematoxylin & eosin (H&E) staining of gastrocnemius, soleus, TA, and EDL muscle of Spin1 M5 and control mice at 30 weeks of age. ( f ) Fiber types in glycolytic (white) or oxidative (red) parts of the TA of Spin1 M5 and control mice at 15 weeks of age (top and middle rows) observed by immunofluorescence (IF) staining. Tissue sections were stained with selective antibody directed against MHC-I (purple), MHC-IIb (cyan), MHC-IIa (red), and MHC-IIx (green). For comparison, NADH staining was included (bottom row). Fibers with abnormal NADH staining are marked with arrows. Corresponding fibers in each column of images are squared

Journal: Cell Death & Disease

Article Title: The histone code reader Spin1 controls skeletal muscle development

doi: 10.1038/cddis.2017.468

Figure Lengend Snippet: Surviving Spin1 M5 mice exhibit major defects in soleus, tibialis anterior, and diaphragm. ( a,b ) Appearance ( a ) and average body weight ( b ) of Spin1 M5 and control mice ( n =5 females in each category) at 30 weeks of age. Error bars represent +S.D., ** P <0.01. ( c ) Degeneration of the soleus (SOL) muscle in adult Spin1 M5 compared with control mice exemplified at 16 weeks of age. ( d ) Hind limb muscles (gastrocnemius (GC), plantaris (PL), soleus, tibialis anterior (TA), extensor digitorum longus (EDL), and quadriceps (QC)) of Spin1 M5 and control mice at 30 weeks of age. Arrows point at the soleus embedded in gastrocnemius and plantaris, which is visible in control but degenerated in Spin1 M5 mice. ( e ) Hematoxylin & eosin (H&E) staining of gastrocnemius, soleus, TA, and EDL muscle of Spin1 M5 and control mice at 30 weeks of age. ( f ) Fiber types in glycolytic (white) or oxidative (red) parts of the TA of Spin1 M5 and control mice at 15 weeks of age (top and middle rows) observed by immunofluorescence (IF) staining. Tissue sections were stained with selective antibody directed against MHC-I (purple), MHC-IIb (cyan), MHC-IIa (red), and MHC-IIx (green). For comparison, NADH staining was included (bottom row). Fibers with abnormal NADH staining are marked with arrows. Corresponding fibers in each column of images are squared

Article Snippet: For immunofluorescence staining the following primary antibodies were used: SPIN1(5865) 1 μ g/ml; Pax7 (PAX7, DSHB, batch 7/2/15) 2 μ g/ml; Tcf4 (6H5-3, Millipore, Darmstadt, Germany, 05-511, batch 2155406) 10 μ g/ml; Ankrd1 (Proteintech Group, Manchester, UK, 11427-1-AP, batch 1951) 1:100; Ankrd2 (Proteintech Group, 11821-1-AP, batch 7649) 1:100; dystrophin (Abcam, Cambridge, UK, ab15277, batch GR226781-6) 1:500; MHC-I (NOQ7.5.4D, Sigma, Munich, Germany, M8421, batch 035M4792V) 1:2000; MHC-IIa (SC-71, DSHB, batch 4/7/16) 1:10; MHC-IIx (6H1, DSHB, batch 3/3/16) 1:6; MHC-IIb (BF-F3, DSHB, batch 5/12/16) 1:20; MCH, skeletal, fast (MY-32, Sigma, M4276, batch 083M4790V) 1:1000; GNZ (anti-GFP, Abcam, ab13970, batch GR236651) 1:1000; normal rabbit IgG (Santa Cruz, Heidelberg, Germany, sc-2027).

Techniques: Control, Muscles, Staining, Immunofluorescence, Comparison

(A) Morphology of the gastrocnemius of wild-type (WT) and HSA-NRTN mice. (B) Weight of soleus, extensor digitorum longus (EDL), gastrocnemius, and tibialis anterior (TA) muscles (n = 9-12) normalized by body weight. (C) Histology of WT and HSA-NRTN TA muscles as determined by hematoxylin and eosin staining. (D and E) Fiber cross-sectional area (CSA) distribution (D) and average (E) in TA muscles of WT and HSA-NRTN mice (n = 5-7). (F and G) Immunohistochemical analysis of fiber type composition in TA muscles of WT and HSA-NRTN mice. Cell membranes are visualized using wheat germ agglutinin (WGA, in red). Representative microscopy images (F) and the corresponding quantification (G) are shown. (H) MyHC-IIx and MyHC-IIb protein levels in gastrocnemius muscles of WT and HSA-NRTN mice. (I) Gene expression of calcium handling machinery components. The graph shows the relative expression of genes involved in calcium handling in gastrocnemius muscles of wild-type and HSA-NRTN mice. Each gene is annotated as enriched in fast muscle (EDL) or slow muscle (soleus) based on published gene array data comparing mouse EDL and soleus muscles (n = 6). Bars depict mean values and error bars represent SEM. * p<0.05, ** p<0.01, *** p<0.001. See also Figure S2 .

Journal: bioRxiv

Article Title: Muscle-secreted neurturin couples myofiber oxidative metabolism and slow motor neuron identity

doi: 10.1101/2021.03.31.437883

Figure Lengend Snippet: (A) Morphology of the gastrocnemius of wild-type (WT) and HSA-NRTN mice. (B) Weight of soleus, extensor digitorum longus (EDL), gastrocnemius, and tibialis anterior (TA) muscles (n = 9-12) normalized by body weight. (C) Histology of WT and HSA-NRTN TA muscles as determined by hematoxylin and eosin staining. (D and E) Fiber cross-sectional area (CSA) distribution (D) and average (E) in TA muscles of WT and HSA-NRTN mice (n = 5-7). (F and G) Immunohistochemical analysis of fiber type composition in TA muscles of WT and HSA-NRTN mice. Cell membranes are visualized using wheat germ agglutinin (WGA, in red). Representative microscopy images (F) and the corresponding quantification (G) are shown. (H) MyHC-IIx and MyHC-IIb protein levels in gastrocnemius muscles of WT and HSA-NRTN mice. (I) Gene expression of calcium handling machinery components. The graph shows the relative expression of genes involved in calcium handling in gastrocnemius muscles of wild-type and HSA-NRTN mice. Each gene is annotated as enriched in fast muscle (EDL) or slow muscle (soleus) based on published gene array data comparing mouse EDL and soleus muscles (n = 6). Bars depict mean values and error bars represent SEM. * p<0.05, ** p<0.01, *** p<0.001. See also Figure S2 .

Article Snippet: Fiber typing was performed with Development Studies Hybridoma Bank (DSHB, Iowa, USA) mouse monoclonal antibodies for MyHC-I (BA-D5), MyHC-IIa (2F7), MyHC-IIx (6H1) and MyHC-IIb (BF-F3).

Techniques: Staining, Immunohistochemical staining, Microscopy, Expressing